Determination of Choline in Infant Formula by Ion Chromatography


Choline is a water-soluble micronutrient vital to cell membrane integrity, support of methyl group metabolism, and nervous system activity. It is present as free choline in small quantities in a wide variety of foods and frequently found in its esterified forms. Choline can also be found in fortified foods and dietary supplements; e.g., choline is a required additive in many infant formulas. It is therefore important to determine the choline content of common foods.

AOAC Official Method 999.14 describes the determination of choline in infant formula and milk using an enzymatic colorimetric method. Thermo Scientific Application Note (AN) 124 and Application Update (AU) 189 demonstrate the determination of choline in infant formula and other foods by ion chromatography (IC).

This webinar will discuss the improvements made to the IC method for the determination of total choline (free and bound forms) in all forms of infant formula/adult nutritionals/pediatric formulas (powder, RTF, and liquid concentrates). The Reagent-Free™ IC (RFIC™) system requires only a source of degassed DI water for generation of high-purity eluent, simplifying operation while increasing precision and accuracy. Suppressed conductivity detection allows simple and robust determination of choline in different samples with high sensitivity. In addition, the use of the microwave to complete the hydrolysis of bound choline to free choline significantly decreases the sample preparation time.

Key Learning Objectives:

  • AOAC standard method performance requirements for choline in infant formula
  • Principles of electrolytic eluent generation
  • Sample preparation using microwave assisted hydrolysis and the use of OnGuard II Cartridges

Who Should Attend:

  • Analytical chemists who want an additional understanding of Ion Chromatography with Suppressed Conductivity Detection in food samples
  • Product Development Scientists who need to become knowledgeable about methods for studying and identifying cations in milk products.
  • Quality Control and Regulatory Affairs professionals in the food industry
Kassandra Oates
Applications Chemist, Marketing
Thermo Fisher Scientific
View Bio

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