AB Sciex

The Power of Accurate Mass MS/MS Data Acquisition Using LC-Tandem Mass Spectrometry over High Resolution Full Scan Mass Measurement in Targeted and Non-Targeted (STA) Toxicological Analysis

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Combining Accurate Mass Measurement with MS/MS Library Searching for Targeted and General Unknown Toxicological Screening

Liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) has become increasingly important in the fields of clinical and forensic toxicology as well as in doping control monitoring. Over the last 5 years or so, thanks to commercially available Enhanced Product Ion (EPI) spectral libraries, methods based on using hybrid triple quadrupole linear ion trap (QTRAP) instruments have been widely deployed for multi-target screening and/or quantification of drugs, poisons and their metabolites in various biomatrices.

More recently, qualitative screening approaches based on high-resolution full scan, accurate mass measurement of precusrsor ion have been deployed for both targeted screening with the added benefit of allowing retrospective data analysis for investigational purposes. However, in complex biological matrices, this approach, without including MS/MS product ion measurements can lead to erroneous assignment of the intoxicant ID.

In this webinar, we will show how the AB SCIEX Triple ToF LC/MS/MS systems simultaneously acquire both full scan MS and MS/MS accurate mass data at unprecedented speeds to enable both quantitative and qualitative analysis to be performed in a single injection on UHPLC time scales with the highest degree of confidence in assigning the correct intoxicant ID.

Particular attention will be focused on a unique accurate mass LC-MS/MS workflow: General Unknown Comparative Screening (GUCS), for the identification of completely unknown drugs and their metabolites in biological matrices. This GUCS workflow rapidly allows identification of unknown / unexpected intoxicants in human biomatrices without the requirement for prior knowledge about possible identity of the intoxicant. Due to the specificity /selectivity of the MS/MS data acquired in the above workflow, it is particularly suitable for the in-depth analysis of real life, complex samples, where matrix interferences are often experienced.


Dr. Adrian Taylor
Technical Marketing Manager
AB SCIEX, Canada
View Bio
Liz Perill
Elsevier Publisher

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