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This webinar is designed to discuss current trends of flow cytometry technologies with regard to a variety of experimental processes in the laboratory. Using a complex technology such as flow cytometry effectively demands attention to a number of instrument and experimental design issues. Fortunately, there are many resources available to assist users in understanding the limitations and opportunities of the technology. In this presentation, Drs. J. Paul Robinson (Purdue University) and John P. Nolan (La Jolla Bioengineering Institute) will discuss some of the calibration and standardization approaches that will ensure that the experimental data obtained is accurate and readily interpreted by other researchers. In addition, expanding assays from a few tubes to large plate arrays demands a rethinking of the experimental design, execution and data analysis. Finally, with the increased use of cell sorting, many users may not be aware of the important issues involved in appropriate and acceptable safety involved in sorting viable human cells, or cells containing infectious agents. Essential elements of each of these topics will be discussed and additional resources available to flow cytometry users will be highlighted.

Drs. Robinson and Nolan will answer your questions live during the webinar so be sure to attend and get answers from the experts!

What you will learn in this webinar:

  • Essential concepts in flow cytometry instrument and assay calibration and standardization
  • Crucial considerations for expanding basic flow assays to larger scale and higher throughput
  • Cell sorting safety practices
  • An overview of Current Protocols in Cytometry coverage across many different fields of science

Who should attend:

  • Researchers who need to develop greater expertise in using flow cytometry
  • Faculty interested in evaluating current flow cytometry practice and opportunities
  • Technical staff who are new or experienced operators
  • Compliance staff at institutions
Dr. J. Paul Robinson
SVM Professor of Cytomics and Professor of
Biomedical Engineering, Purdue University
View Presenter Biography
Dr. John P. Nolan
International Society for Advancement of Cytometry
View Presenter Biography

Flow Cytometry: Essential Instrument and Experimental Design Considerations

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