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Fluorescence microscopy imaging has been widely used in neuroscience and cancer research, but it still has two limitations: (1) The number of fluorophores that can be simultaneously imaged in a single staining and imaging round is limited to 10, and (2) it is not easy to achieve super-resolution imaging over the whole organism.
In this webinar, Dr. Jae-Byum Chang presents two state-of-the-art techniques that he has recently developed. The first technique is PICASSO, which enables greater than 10-color multiplexed imaging of specimens without any reference spectra measurements, even with microscopy equipped with emission filters. The second technique is whole-body expansion microscopy, which enables the super-resolution imaging of all anatomical structures of whole vertebrates, especially zebrafish larvae.