PICASSO and whole body-expansion microscopy for ultra-multiplexed imaging and whole-body super-resolution imaging

Session 2:
Friday, September 24, at 16:00 BST / 17:00 CEST / 11:00 EDT / 08:00 PDT
Register for this event
Session 2:
Friday, September 24, at 16:00 BST / 17:00 CEST / 11:00 EDT / 08:00 PDT
Register for this event

Sign Up:

(*) denotes required form field(s)

Our registration process uses cookies, by submitting this registration form you agree to our cookie policy.

  Register

Overview

Facebook icon Twitter iconLinkedIn icon

Fluorescence microscopy imaging has been widely used in neuroscience and cancer research, but it still has two limitations: (1) The number of fluorophores that can be simultaneously imaged in a single staining and imaging round is limited to 10, and (2) it is not easy to achieve super-resolution imaging over the whole organism. 

In this webinar, Dr. Jae-Byum Chang presents two state-of-the-art techniques that he has recently developed. The first technique is PICASSO, which enables greater than 10-color multiplexed imaging of specimens without any reference spectra measurements, even with microscopy equipped with emission filters. The second technique is whole-body expansion microscopy, which enables the super-resolution imaging of all anatomical structures of whole vertebrates, especially zebrafish larvae.

Learning objectives:

  • Understand the limitations of the current multiplexed imaging techniques
  • Learn how ultra-high multiplexed imaging can be used to study the molecular heterogeneity of the brain
  • Discover the advantages of whole-body super-resolution imaging of vertebrates
Dr. Jae-Byum Chang
Assistant professor, Department of Materials Science and Engineering
Korea Advanced Institute of Science and Technology (KAIST)