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Developing a spatio-temporal single-cell type map of adult human tissues

Available on demand

To comprehensively understand human health and applications of molecular and precision medicine, it is critical to characterize biological processes at the tissue and cellular level. During this webinar, Dr. Cecilia Lindskog, research group leader and associate professor from Uppsala University, will describe how scRNA-seq, spatial proteomics, and machine learning algorithms can be used to generate high-resolution spatio-temporal maps of human tissues.

Using these approaches, distinct subpopulations of cells linked to pathways involved in normal and disease states were identified. Through a large-scale multiplex immunofluorescence pipeline, deep characterization of >500 proteins was performed to map the spatial localization. Combined analysis of mRNA and protein expression enabled the mapping of temporal and dynamic changes in gene expression and mRNAs that exhibited variable spatio-temporal expression patterns. Furthermore, putative functions were assigned to numerous uncharacterized proteins within a tissue-specific context.

Key learning objectives

Learn about the progress made towards a complete spatial map of the human proteome
Understand how quantitative data can be linked with tissue morphology to create a spatio-temporal single-cell type map
Discover examples of novel cell state-specific expression patterns validated with spatial biology techniques
Explore how spatial biology has provided valuable insights into molecular function and mechanisms of disease pathways

Who should attend?

Researchers
Principal investigators
Professors
Post-doctoral researchers

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Speakers:

Dr. Cecilia Lindskog

Research Group Leader and Associate Professor
Department of Immunology, Genetics and Pathology, Cancer Precision Medicine Unit, Uppsala University, Sweden
View Biography

Georgina Wynne Hughes

Editorial Team
SelectScience
View Biography